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Rapid Spectophotometric Determination Of Sulphonamide Derivatives With Resorcinol

Author(s): P Nagaraja, K. R Sunitha, R. A Vasantha, H. S Yathirajan

A rapid and simple spectrophotometric method for the determination of sulpha drugs is described. The method is based on the formation of a red coloured azo product by the diazotisation of sulphonamides, sulphamethoxazole, sulphadiazene and sulphacetamide followed by a coupling reaction with resorcinol. Absorbance of the resulting red azo product is measured at 500 nm. Beer's law is obeyed in the concentration range of 0.25-7.0 µg/ml at the wavelength of maximum absorption. The method is successfully employed for the determination of sulphonamides in various pharmaceutical preparations.


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