Abstract

The aim of the study was to develop a simple and economic ultra violet-spectrophotometric technique for determination of guggul from a polyherbal formulation, following international council for harmonisation guidelines. Endorsement of this method was done by using the high-performance liquid chromatography technique. Ratio and ratio derivative spectroscopy were used and later these were validated by high-performance liquid chromatography method. Inter and intra-day precision, % recovery were done for accuracy of the method. Further the methods were employed for the determination of guggul concentration in a formulation. The maximum absorption (λmax) of guggul in aqueous solution was found to be 280 nm. The linearity concentration range of 1-5 mg/ml with satisfactory R2 value of 0.998 was observed. % recovery at three different levels, 80 %, 100 % and 120 % found within the range of 90.90-93.93 %, indicating good accuracy. Furthermore, the low values of % RSD demonstrated the method’s accuracy and reproducibility. The precision of the method was evaluated through intraday and inter-day studies, as well as repeatability. The % RSD value, which was found to be less than 2, indicated the method was precise. The results obtained from the ratio derivative spectroscopy analysis were further analysed using the high-performance liquid chromatography method. The % recovery data of high-performance liquid chromatography method was in good agreement with the % recovery data of the ratio derivative spectroscopy analysis, confirming the reliability of the method. Based on the results it can be concluded that ratio derivative spectroscopy method was comparable with high-performance liquid chromatography method. Ratio derivative spectroscopy method was reliable and precise technique for determining guggul from polyherbal formulations. Hence ratio derivative spectroscopy might be the used as a method for the quality control of guggul from polyherbal formulations in industry as a simple, rapid and economic method compared to high-performance liquid chromatography method.