Abstract
Reverse phase high performance liquid chromatography method for estimation of ezetimibe in bulk and pharmaceutical formulations
Author(s): SK Akmar, Lata Kothapalli, Asha Thomas, Sumitra Jangam, AD Deshpande
Department of Pharmaceutical Chemistry, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune - 411 018, India
Correspondence Address:
Lata Kothapalli Department of Pharmaceutical Chemistry, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune - 411 018 India E-mail: hemanthip@yahoo.co.in
Department of Pharmaceutical Chemistry, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune - 411 018, India
Correspondence Address:
Lata Kothapalli Department of Pharmaceutical Chemistry, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune - 411 018 India E-mail: hemanthip@yahoo.co.in
A rapid, precise, economical and accurate HPLC method for estimation of ezetimibe in bulk and pharmaceutical formulations has been developed. The chromatographic resolution of ezetimibe was achieved using acetonitrile:0.02 M potassium dihydrogen orthophosphate buffer (72:28 v/v) as the mobile phase with UV detection at 232 nm and C8 kromasil 5 μ column. The flow rate was 1 ml/min. Results of the analysis were validated statistically and by recovery studies and were found to be satisfactory.
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