Abstract

Simple, rapid and precise reversed-phase HPLC method has been developed for the quantitation of glimepiride in tablet on a Hypersil C-18 (15 cmx3.9 mm) column using a mobile phase consisting acetonitrile:0.05 M monobasic potassium phosphate (pH 6.0) (40:60 v/v) at a flow rate of 1.5 ml/min and detection at 210 nm. The retention time of glimepiride have been found to be 7.8 min and recoveries were between 99-101%. Validation of the proposed method also been done.