Abstract
Role of Ethanolic Extract of Microdus brasiliensis (Duby) Ther. in Protecting Survival Rate in Sepsis: An In vivo Study
Department of Pharmacology, AU College of Pharmaceutical Sciences, Andhra University, Visakhapatnam-530 003, India, 1Department of Medical Microbiology and Parasitology, Faculty of Medicine, Duy Tan University, Da Nang 550000, Vietnam, 2Center for Molecular Biology, Duy Tan University, Duy Tan University, Da Nang 550000, Vietnam, 3Faculty of Pharmacy, Duy Tan University, Da Nang 550000, Vietnam, 4School of Pharmacy, Centurion University of Technology and Management, Odisha 767001, India
Correspondence Address:
K. N. Killari, Department of Pharmacology, AU College of Pharmaceutical Sciences, Andhra University, Visakhapatnam-530 003, India, E-mail: kishorenaidu.killari@gmail.com
The present study investigated the phytochemical, total flavonoid and total phenolic content, antibacterial activity, acute toxicity studies, hemodynamic parameters, myeloperoxidase activity of ethanolic extract of Microdus brasiliensis and its effects on cecal ligation and puncture induced sepsis in mice. The preliminary phytochemical screening showed that Microdus brasiliensis contains alkaloids, terpenoids, flavonoids, phenolics, saponins and tannins. The Microdus brasiliensis found to contain good amounts of total flavonoid and phenolic content. At equivalent concentration, Microdus brasiliensis showed antibacterial activities as potent as streptomycin against Staphylococcus aureus, Salmonella typhi and Escherichia coli. Acute toxicity studies on mice found out that Microdus brasiliensis was non-toxic up to 2000 mg/kg body weight. At both low and high doses, Microdus brasiliensis improved hemodynamic parameters such as mean arterial pressure and decreased optical density of blood and serum myeloperoxidase activity. Moreover, at a high dose, Microdus brasiliensis reduced survival rate to 80.34±3.67 % in mice that might be through pro-inflammatory effects. The results indicate that Microdus brasiliensis can be a favorable natural source for the treatment of cecal ligation and puncture induced sepsis in mice.
