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Separation and identification of phenolic acid and flavonoids from Nerium indicum flowers

Author(s): A Vinayagam1, PN Sudha2
1Department of Chemistry, Sathyabama University, Chennai-600 119, India 2Department of Chemistry, DKM College for Women, Thiruvallur University, Vellore-632 001, India

Correspondence Address:
A Vinayagam Department of Chemistry, Sathyabama University, Chennai-600 119 India E-mail:

Four major compounds were separated and identified from the methanol extracts of Nerium indicum flowers (Arali) using HPLC and mass spectral data. Through mass data, the chemical structures were elucidated as: trans5-O-caffeoylquinic acid (1), quercetin-3-O- rutinoside (2), luteolin-5-O-rutinoside (3) and luteolin-7-O-rutinoside (4). In addition, the cis isomers of 5-O-caffeoylquinic acid in Nerium indicum flowers were confirmed by Mass, HPLC and UV. The structures of these compounds confirmed with the help of liquid chromatography mass spectrometry.

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