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Abstract

Simultaneous determination of salbutamol sulphate and bromhexine hydrochloride in tablets by reverse phase liquid chromatography

Author(s): P. N. S. Pai, GK Rao, MS Murthy, A Agarwal, S Puranik
Department of Quality Assurance, Al-Ameen College of Pharmacy, Bangalore-560 027, India

Correspondence Address:
P. N. S. Pai Department of Quality Assurance, Al-Ameen College of Pharmacy, Bangalore-560 027 India [email protected]


A simple reverse phase liquid chromatographic method has been developed and subsequently validated for simultaneous determination of salbutamol sulphate and bromhexine hydrochloride. The separation was carried out using a mobile phase consisting of acetonitrile, methanol and phosphate buffer, pH 4 in the ratio 60:20:20 v/v. The column used was SS Wakosil-II C-18 with a flow rate of 1 ml/min and UV detection at 224 nm. The described method was linear over a concentration range of 10-110 µg/ml and 20-140 µg/ml for the assay of salbutamol sulphate and bromhexine hydrochloride, respectively. The mean recovery was found to be 95-105% for salbutamol sulphate and 96.2-102.1% for bromhexine hydrochloride when determined at five different levels.

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