Abstract
Simultaneous determination of valsartan and hydrochlorothiazide in tablets by RP-HPLC
Author(s): DF Tian, XL Tian, T Tian, ZY Wang, FK Mo
Department of Pharmacy, School of Pharmacy, Shenyang Pharmaceutical University, 110016 Shenyang, China
Correspondence Address:
F K Mo Department of Pharmacy, School of Pharmacy, Shenyang Pharmaceutical University, 110016 Shenyang China E-mail: fengkuimo@yahoo.com.cn
Department of Pharmacy, School of Pharmacy, Shenyang Pharmaceutical University, 110016 Shenyang, China
Correspondence Address:
F K Mo Department of Pharmacy, School of Pharmacy, Shenyang Pharmaceutical University, 110016 Shenyang China E-mail: fengkuimo@yahoo.com.cn
A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous determination of valsartan and hydrochlorothiazide in tablets. A column having 200 × 4.6 mm i.d. in isocratic mode with mobile phase containing methanol:acetonitrile:water:isopropylalcohol (22:18:68:2; adjusted to pH 8.0 using triethylamine; v/v) was used. The flow rate was 1.0 ml/min and effluent was monitored at 270 nm. The retention time (min) and linearity range (µg/ml) for valsartan and hydrochlorothiazide were (3.42, 8.43) and (5-150, 78-234), respectively. The developed method was found to be accurate, precise and selective for simultaneous determination of valsartan and hydrochlorothiazide in tablets.
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