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Abstract
Simultaneous Estimation Of Ethinyl Estradiol And Levonorgestrel From Transdermal Patches By HPLC
Author(s): Bala Prabhakar, S. G Deshpande
The present work describes a simple reverse phase HPLC method for the determination of ethinyl estradiol (ETES) and levonorgestrel (LN) from transdermal patches. The determination was carried out on a Phenomenex, C-18, 250 x 4.6 mm, 10 micron column using a mobile phase of acetonitrile/ water (55:45). The flow rate was 1.2 ml/minute. The eluent was monitored at 225 nm. Quantitation was done using an external standard. The method was found to be reproducible, with good resolution between ETES and LN. The detector response was found to be linear in the concentration range of 1- 5 ppm for ETES and 5.25 ppm for LN. The sensitivity of the method was found to be 0.1 mg/ml.
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