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Abstract
Simultaneous HPLC estimation of omeprazole and domperidone from tablets
Author(s): Lakshmi Sivasubramanian1, V Anilkumar2
1 Department of Pharmaceutical Analysis, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur - 603 203, India 2 Department of Pharmaceutical Analysis, Vel's College of Pharmacy, Chennai - 600 043, India
Correspondence Address:
Lakshmi Sivasubramanian Department of Pharmaceutical Analysis, SRM College of Pharmacy, SRM Institute of Science and Technology, India E-mail: lakshmiss@hotmail.com
1 Department of Pharmaceutical Analysis, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur - 603 203, India 2 Department of Pharmaceutical Analysis, Vel's College of Pharmacy, Chennai - 600 043, India
Correspondence Address:
Lakshmi Sivasubramanian Department of Pharmaceutical Analysis, SRM College of Pharmacy, SRM Institute of Science and Technology, India E-mail: lakshmiss@hotmail.com
The present work describes a simple reverse phase HPLC method for the determination of omeprazole and domperidone from tablet formulations. The determination was carried out on a Hypersil, ODS, C-18 (150×4.6 mm, 5 micron) column using a mobile phase of methanol:0.1 M ammonium acetate (pH 4.9) (60:40). The flow rate and runtime were 1 ml/min and 10 min, respectively. The eluent was monitored at 280 nm. The method was reproducible, with good resolution between omeprazole and domperidone. The detector response was found to be linear in the concentration range of 10-60 µg/ml for omeprazole and 5-30 µg/ml for domperidone.
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