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Abstract

Simultaneous HPTLC determination of rabeprazole and itopride hydrochloride from their combined dosage form

Author(s): A Suganthi, Sofiya John, TK Ravi
Department of Pharmaceutical Analysis, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore, India

Correspondence Address:
A Suganthi Department of Pharmaceutical Analysis, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore-641 044 India E-mail: [email protected]


A simple, precise, sensitive, rapid and reproducible HPTLC method for the simultaneous estimation of the rabeprazole and itopride hydrochloride in tablets was developed and validated. This method involves separation of the components by TLC on precoated silica gel G60F254 plate with solvent system of n-butanol, toluene and ammonia (8.5:0.5:1 v/v/v) and detection was carried out densitometrically using a UV detector at 288 nm in absorbance mode. This system was found to give compact spots for rabeprazole (Rf value of 0.23 0.02) and for itopride hydrochloride (Rf value of 0.75±0.02). Linearity was found to be in the range of 40-200 ng/spot and 300-1500 ng/spot for rabeprazole and itopride hydrochloride. The limit of detection and limit of quantifi cation for rabeprazole were 10 and 20 ng/ spot and for itopride hydrochloride were 50 and 100 ng/spot, respectively. The method was found to be benefi cial for the routine analysis of combined dosage form.

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