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Spectrophotometric Assay Methods for Determination of Amiloride: A Charge-Transfer Reaction Approach with 2,3-Dichloro-5,6-Dicyano-1,4-Benzoquinone and p-Chloranilic Acid Reagents

Author(s): P. C. Hanamshetty*, K. Siddappa, P. Chavan and M. M. Nagabhushana
Department of Chemistry, Guru Nanak First Grade College, Bidar, Karnataka 585402, 1Department of Studies and Research in Chemistry, Gulbarga University, Gulbarga, Karnataka 585106, 2Department of Post-Graduate Studies and Research in Chemistry, Sahyadri Science College, Kuvempu University, Shivamogga, Karnataka 577203, 3Department of Chemistry Government Engineering College, Huvinahadagali, Karnataka 583219, India

Correspondence Address:
P. C. Hanamshetty, Department of Chemistry, Guru Nanak First Grade College, Bidar, Karnataka 585402, India, E-mail:

The present study proposes two straightforward, responsive and inveterate spectrophotometric techniques for the detection of amiloride hydrochloride. The methodologies are mainly relay on the generation of charge transfer complex, with the reagents 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (Method A) and p-chloranilic acid (Method B), hence an enduring 1:1 stoichiometric complex were accomplished, which are yellow and pink colour correspondingly. The generated chromogens optical densities were measured at 476 and 508 nm respectively. Beer’s law been tested in the range of 5-30 μ and 1-6 μ The other analytical parameters like, Limit of Detection (LOD), Limit of Quantification (LOQ), accuracy and precisions were examined. The mentioned approaches were successfully adopted for the determination of the amiloride hydrochloride in tablet formulation with high accuracy, better recoveries and acceptable relative standard deviation.

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