Spectrophotometric determination of amino acids and their dosage forms.
A.R. College of Pharmacy, Vallabh Vidyanagar-388 120,
S A Shah A.R. College of Pharmacy, Vallabh Vidyanagar-388 120
A spectrophotometric method for the estimation of Lysine hydrochloride and Glumatic acid based on their reaction with acetylacetone-formaldehyde reagent is reported. The resulting colored chromogenic species in solution is directly measured at their wavelength of maximum absorbance 415 nm. Beer's Law is obeyed in concentration range 10-60 mcg/ml of Lysine hydrochloride and 20-160 mcg/ml of glumatic acid. The proposed method is applied to analyse their pharmaceutical formulations. The results compare favourably with those obtained by official method.PDF