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Spectrophotometric estimation of cefotaxime and ceftriaxone in pharmaceutical dosage forms

Author(s): SA Patel, NM Patel, MM Patel

A simple, sensitive, accurate and rapid spectrophotometric method has been developed for the estimation of cefotaxime and ceftriaxone using Folin-Ciocalteu reagent in presence of 20% sodium carbonate solution. The blue colour chromogen formed is measured at wavelength of maximum absorption 752 nm and 750 nm for cefotaxime and ceftriaxone respectively against reagent blank. The chromogen obeyed linearity over 5.0 to 60 µg/ml for cefotaxime, and 2.0 to 36 µg/ml for ceftriaxone. The results of analysis have been validated statistically and by recovery studies.

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