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Spectrophotometric Estimation Of Itraconazole In Pharmaceutical Formulations

Author(s): T. K Murthy, M. N Reddy, Y Srinivasa Rao, D. G Sankar

Two simple spectrophotometric methods (A and B) have been developed for the determination of itraconazole in pure and in its pharmaceutical formulations. Method A is based on the formation of blood red colored complex with ferric chloride and 1,10-phenanthroline having absorption maximum at 520 nm, where as in method B, itraconazole forms a green colored complex with ferric chloride and MBTH reagent exhibiting maximum absorption at 630 nm .The chromogens obey Beer's law in the concentration ranges of 1.2 to 7.5 μg/ml and 2.5 t o 20 μg/ml for methods A and B, respectively. The results obtained are reproducible and are statistically validated.


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