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Spectrophotometric Estimation Of Melatonin And Meloxicam Using Folin-Ciocalteu Reagent

Author(s): B. S Kuchekar, S. G Late, A. A Shingavi, D. B Shinde

A simple, accurate, rapid and sensitive method has been developed for the estimation of melatonin and meloxicam using Folin-Ciocalteu reagent in the presence of 20% sodium carbonate solution. The blue coloured chromogen formed is measured at wavelength of maximum absorption 700 nm against reagent blank. The chromogen obeyed linearity over 1.5 to 15 μg/ml for melatonin and 1.5 to 22.5 μg/ml for meloxicam.