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Studies On Phospholipids Of Some Linseed Varieties

Author(s): A. S Kulkarni, R. R Khotpal, H. A Bhakare, P. B Shingwekar

Five varieties of Linseed (Linum usltatissimum) cultivated in the Vidarbha region, namely, C-429, R-552, RLC-4, RLC-6 and T-397, were extracted with chloroform-methanol (2:1, v/v) to get the total lipids (TL) which were then fractionated over a silicic acid column into neutral lipids (NL), glycolipids (GL) and phospholipids (PL). The PL were further separated into individual components namely, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), cardiolipin (CL) and phosphatidylglycerol (LPG) by preparative TLC. The fatty acid composition of PL and its components, as determined by GLC, showed that linolenic acid was the predominant acid present, followed by oleic, linoleic, palmitic and stearic acids. The fatty acids remained constant qualitatively but differed quantitatively in the seed oils and phospholipids.


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