Study on stem callus of solanum platanifolium sims.
Dept. of Pharmaceutical Sciences, Panjab University, Chandigarh 160 014,
R K Jaggi Dept. of Pharmaceutical Sciences, Panjab University, Chandigarh 160 014
Callus cultures were established from the stem explants of the plant Solanum platanifolium Sims. Three media, namely BW, BMS and BMSKS were used for the production of the maximum biomass of callus. Effect of IAA, NAA and 2,4-D alone as well as in combination with Kn was observed on the induction and further growth of callus, Optimum yield of callus was obtained on BMS + 2,4-D (1-2 ppm) + Kn (0.1-5 ppm) and BMS + NAA (10 ppm) + Kn (1-5 ppm) in the presence of light. GC-MS analysis of the grown calli showed the presence of Beta-sitosteral and lupeol. The different growth regulators produced the same pattern of secondary metabolites in the calli.