Abstract
The binding interactions of the macrolide endectocide ivermectin with the antibiotics ampicillin, chloramphenicol and tetracycline HCL
1United Graduate School of Drug Discovery and Medical Information Sciences,Gifu University, Japan 2Kafrelshikh University Veterinary Hospital, Faculty of Veterinary Medicine, Kafrelshikh University, Egypt 3Department of Biomolecular Science,Faculty of Engineering, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan
Correspondence Address:
M Kandeel United Graduate School of Drug Discovery and Medical Information Sciences,Gifu University, Gifu 501-1193 Japan E-mail: mahmoud.kandeal@vet.kfs.edu.eg
Ivermectin, chloramphenicol, ampicillin and tetracycline HCl are common drugs in human and veterinary practice. The purpose of this study is to investigate the possible binding interactions between ivermectin and the antibiotics chloramphenicol, ampicillin and tetracycline HCl. Isothermal titration calorimetry was used to determine the binding interactions between ivermectin and these antibiotics. Results indicated that, about three molecules of ampicillin can bind to one molecule of ivermectin and about one molecule of chloramphenicol with one molecule of ivermectin. However, no binding stoichiometry can be detected with tetracycline HCl-ivermectin titration. Furthermore, the binding interactions were accompanied by various biophysical and biochemical mechanisms. This is the first report of such interactions of ivermectin with chloramphenicol, ampicillin and tetracycline HCl. There are possible binding interactions of ivermectin with chloramphenicol and ampicillin. Further studies are required for detecting the impact of this binding on biological aspects of drug actions.
