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Abstract

Tissue Culture Studies Of Centella Asiatica

Author(s): K Purushotham Rao, S Srinath Rao, M Sadanandam

To meet the growing need of drugs, research and development scientists now concentrate on the development of herbal formulations. In the present investigation, the influence of auxins and cytokinins during the production of callus in Centella asiatica (L) was studied. The work plan has been carried out using known procedures of tissue culture under specified conditions of inoculation and incubation in Murashige and Skoogs medium. It was noticed that stem explant, stolon proved to be the best for callus induction followed by leaf, base, kinetin supplementation at 0.25 and 0.5 mg/l along with auxins 2 mg/l 2,4- D proved to be beneficial for the growth of callus. The best combinations of growth regulators for maximum callus induction was 2 mg/l NAA+0.5 mg/l kinetin.

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