All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Tramadol Suppresses the Proliferation, Migration and Invasion of SNU-1 Gastric Cancer Cells by regulating the microRNA-212-5p/Wnt/β-Catenin Pathway

Author(s): Ping Cao, Xiaojun Wang and Yanping Zhang*
Department of Gastroenterology, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan 030032, China

Correspondence Address:
Yanping Zhang, Department of Gastroenterology, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan 030032, China, E-mail: caoping842@126.com


The study includes the function of tramadol on the oncogenic phenotypes of SNU-1 gastric cancer cells and its mechanism. SNU-1 cells were divided into control group, tramadol group, microRNA-negative control group, microRNA-212-5p group, 300 μg/ml tramadol+anti-microRNA-negative control group and 300 μg/ ml tramadol+anti-microRNA-212-5p group. SNU-1 cell proliferation, invasiveness and migration were quantified. Reverse transcription-quantitative polymerase chain reaction and Western blot examined the microRNA-212-5p and beta-catenin expression. The proliferation, invasiveness and migration of SNU-1 cells in tramadol (75 μg/ml, 150 μg/ml or 300 μg/ml) group were significantly reduced. Tramadol elevated microRNA-212-5p level in SNU-1 cells, and microRNA-212-5p elevation suppressed SNU-1 cell proliferation, migration and invasion. Moreover, microRNA-212-5p deficiency reversed the anticancer activity of tramadol in SNU-1 cells. Additionally, tramadol declined beta-catenin protein in SNU-1 cells, while microRNA-212- 5p deficiency abolished this effect of tramadol. Tramadol inhibited SNU-1 gastric cancer cell proliferation, invasiveness and migration by the microRNA-212-5p/Wnt/β-catenin pathway.

Full-Text | PDF

 
 
Google scholar citation report
Citations : 69022

Indian Journal of Pharmaceutical Sciences received 69022 citations as per google scholar report