Abstract
Tramadol Suppresses the Proliferation, Migration and Invasion of SNU-1 Gastric Cancer Cells by regulating the microRNA-212-5p/Wnt/β-Catenin Pathway
Department of Gastroenterology, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan 030032, China
Correspondence Address:
Yanping Zhang, Department of Gastroenterology, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan 030032, China, E-mail: caoping842@126.com
The study includes the function of tramadol on the oncogenic phenotypes of SNU-1 gastric cancer cells and its mechanism. SNU-1 cells were divided into control group, tramadol group, microRNA-negative control group, microRNA-212-5p group, 300 μg/ml tramadol+anti-microRNA-negative control group and 300 μg/ ml tramadol+anti-microRNA-212-5p group. SNU-1 cell proliferation, invasiveness and migration were quantified. Reverse transcription-quantitative polymerase chain reaction and Western blot examined the microRNA-212-5p and beta-catenin expression. The proliferation, invasiveness and migration of SNU-1 cells in tramadol (75 μg/ml, 150 μg/ml or 300 μg/ml) group were significantly reduced. Tramadol elevated microRNA-212-5p level in SNU-1 cells, and microRNA-212-5p elevation suppressed SNU-1 cell proliferation, migration and invasion. Moreover, microRNA-212-5p deficiency reversed the anticancer activity of tramadol in SNU-1 cells. Additionally, tramadol declined beta-catenin protein in SNU-1 cells, while microRNA-212- 5p deficiency abolished this effect of tramadol. Tramadol inhibited SNU-1 gastric cancer cell proliferation, invasiveness and migration by the microRNA-212-5p/Wnt/β-catenin pathway.
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