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Abstract

Validated Bio-Analytical Method for Cinacalcet Concentrations in Rat Plasma by Reversed Phase-High Performance Liquid Chromatography- Photodiode Array Detector Method

Author(s): N. Mallikarjunarao* and I. B. Mahammad
Department of Pharmaceutical Sciences, Jawaharlal Nehru Technological University Kakinada, Kakinada, Andhra Pradesh 533003, India, 1Department of Pharmacy, University of Technology and Applied Sciences, Muscat 133, Sultanate of Oman

Correspondence Address:
N. Mallikarjunarao, Department of Pharmaceutical Sciences, Jawaharlal Nehru Technological University Kakinada, Kakinada, Andhra Pradesh 533003, India, E-mail: mallimpharmmba@gmail.com


The goal of this work was to create and verify a simple reversed phase-high performance liquid chromatography-photodiode array detector-based method for quantifying cinacalcet in rat plasma. Six albino Wistar rats were given cinacalcet orally by oral gavage. Blood was obtained at regular intervals using retro-orbital vein puncture and processed to get plasma. Cinacalcet was extracted from plasma using a 70:30 v/v ethanol:dichloromethane combination, with an 82.80 %-104.08 % recovery rate. The reversed phase-high performance liquid chromatography-photodiode array detector technique was used to determine cinacalcet plasma concentrations. The mobile phase was acetonitrile:0.1 M dipotassium phosphate buffer (pH 6.8) (50:50 v/v) at a flow rate of 1.0 ml/min. 241.0 nm was used as the detecting wavelength. The method's linearity for cinacalcet was proven by a correlation coefficient (R2) of more than 0.9996 and a linear range of 1 μg/ml to 24 μg/ml for cinacalcet. The lower and upper limits of quantification were found to be 1 μg/ml and 24 μg/ml respectively. At the cinacalcets retention time, no interfering peaks were seen. This reversed phase-high performance liquid chromatography-photodiode array detector approach is a straightforward and effective way to determine pharmacokinetic parameters by measuring plasma cinacalcet levels.

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