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Abstract
Visible Spectrophotometric Methods For The Determination Of Azithromycin In Tablets
Author(s): Lakshmi Sivasubramanian, M Arul Mervin, L Jayashankar, P Ramu, T. K Raja
Two visible spectrophotometric methods have been developed for the estimation of azithromycin in pure and in pharmaceutical formulations. The first method (A), a visible spectrophotometric method was based on the formation of a red coloured chromogen with ferric chloride and 1,10-phenanthroline, which showed absorbance maximum at 490 nm and Beer's law was obeyed in the concentration range of 2.5-15 μg/ml. The second method (B) was based on the formation of a blue coloured chromogen with Folin-Ciocalteau reagent, which showed maximum absorbance at 720 nm and Beer's law was obeyed in the concentration range of 25-150 μg/ml. Results of analysis for both the methods were validated statistically and by recovery studies.
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