- *Corresponding Author:
- G. Manikandan
Department of Biology, The Gandhigram Rural Institute (Deemed to be University) Dindigul, Tamil Nadu- 624001, India
E-mail: [email protected]
|Date of Received||31 July 2020|
|Date of Revision||24 October 2020|
|Date of Acceptance||16 February 2021|
|Indian J Pharm Sci 2021;83(1):45-51|
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In the present investigation, iron nanoparticles were synthesized by using mushroom extract. The production of iron nanoparticles from P. florida was initially confirmed by color changes from brown to dark brown within 30 min at 60° and further characterized by Ultraviolet-Visible spectroscopy and scanning electron microscope with Edax. The biosynthesized iron nanoparticles were subjected to antimicrobial activities and showed higher zone of inhibition against Candida glabrata followed by Micrococcus mucilaginosus, Pseudomonas aeruginosa, Candida albicans, Klebsiella terrigena, K. pneumoniae, Escherichia coli, Candida sp., Bacillus cereus and Staphylococcus aureus. This extract was confirmed as effective against microorganisms which was well comparable with standard antibiotics. The present study has recommended that iron nanoparticles from mushrooms have great potential to act as safe alternative to antibiotics to fight the challenges of drug discovery.
Pleurotus florida, iron nanoparticles, ultraviolet-visible spectroscopy, scanning electron microscope, antimicrobial
Nanotechnology provides the tools and technology platform for the investigation and transformation of biological systems and biology offers inspiration models and bio-assembled components to nanotechnology. Nano biotechnology is a field that applies the nanoscale principle and techniques to understand and transform biosystems (living and non -living) which uses biological principles and materials to create new devices and systems integrated from the nanoscale. Nanotechnology has dynamically developed as an important field of modern research with potential effects in electronic and medicine[2,3,4]. Nanobiotechnology combines biological principles with physical and chemical procedures to generate nano-sized particles with specific functions. Nanobiotechnology represents an economic alternative for chemical and physical methods of nanoparticles formation. These methods of synthesis can be divided on intra cellular and extracellular.
Iron is an essential element which involved in synthesis of many important cell components such as adenine triphosphate (ATP), transport of oxygen, deoxyribonucleic acid (DNA) and electron transport. In human, iron is a component of hemoglobin and myoglobin but when it is low in supply, it leads to nutritional deficiency called, anemia. Anemia is one of the major public health problems in under-developing and developing countries, particularly among pregnant women, nursing mothers and the newborn babies. Iron is also one of the most abundant metals on the earth but it is not readily available. Although, most of the microbes have developed various regulated system for iron uptake, utilization and storage but some food crops show low absorption capability for iron. The diminishing quantity of iron in food has tremendously contributed to increase in malnutrition which therefore required a reliable and alternative source of iron supply in foods to enhance the promotion of good health.
Iron oxide nanoparticles have attracted intensive research interest because of their important applications in cancer therapy, drug delivery, magnetic resonance imaging (MRI) and waste water treatment. The biosynthesis of iron oxide nanoparticles of different sizes and shapes has been reported using bacteria, fungi and plant extract. The bioreduction of metal by combinations of biomolecules found in plant extract, resulting in the formation of metal nanoparticles has been extensively reviewed. The synthesis of nanoparticles using plant extract could be advantageous over other biological processes since it eliminates the elaborate process of maintaining cell cultures.
In recent years, novel size-dependent physicochemical properties have led to metallic iron nanoparticles of great potential in a wide range of applications, including magnetic storage media, ferrofluids, biosensors, catalysts, separation processes and environmental remediation. Specifically, magnetite (Fe3O4) is a common magnetic iron oxide having a cubic inverse spinel structure. The compound exhibits unique electric and magnetic properties based upon the transfer of electrons between Fe2+ and Fe3+ in octahedral sites.
For a long time in Asian countries, a variety of edible mushroom have been taken as vitamin and mineral supplements. Studies on edible mushroom have revealed, valuable activities related to biological response modification. Chemo-preventative, chemotherapeutic, immune modulatory, hypoglycemic and hypocholesterolemic effects have been observed in edible mushroom diets high in mushrooms were reported. Suppress the enzyme aromatase and the active antitumor material was suggested to be a polysaccharide. Mushrooms have variety of bioactive compounds with various biological activities. The content and bioactivity of these compounds depend on the preparation and consumption of mushroom. It is well known that mushrooms are rich in proteins, vitamins and amino acids. Edible mushrooms have antitumor, anti-inflammatory, cardiovascular, antiviral and antibacterial activities[11,12]. Several mushrooms are used for medicinal purposes which accumulate a variety of secondary metabolites including rich sources of beta glucan, proteoglucan, lectin, phenolic compounds, flavonoids, triterpenoids, dietary fiber, leutinan, steroids, glycopeptides, terpenes, saponin and phenolic compounds. All the reported compounds have high therapeutic values and promote health being as agent with antioxidant, antimicrobial, cholesterol lowering and immune stimulatory effect, antifungal and antitumor properties. The synthesis of iron nanoparticles from mushroom and production of new dietary supplements and new pharmaceutical products have been exploited. This study has attempted synthesize bionanoparticles using oyster mushroom and analyzed their antibacterial and antifungal properties. The confluence of nanotechnology and biology could address several biomedical, health and medicine.
Materials and Methods
Collection and preparation of mushroom extracts:
The fruiting bodies of Pleurotus florida were collected from the local market, Chinnalapatti, Dindigul, Tamil Nadu, India. The collected mushrooms were thoroughly washed with tap water and finely cut (1 inch size) and again washed with distilled water. A total of 20 g of fresh mushrooms were added to 100 ml of deionised water and warmed for one hour in a water bath at 60° and filtered through Whatman No. 1 filter paper. The extracts were stored at 4°C for further experiments (Fig. 1A).
Biosynthesis and Characterization of Iron nanoparticles:
A total of 50 ml of mushroom extracts was added to 50 ml of 1 M Ferric Chloride in 1:1 proportion at a temperature of about 50-60° and the solution was stirred continuously. The color change from brown to dark red was noticed and formation dark brown color after certain time period has indicated the formation of iron nanoparticles (Fig. 1-B). After that, the synthesized extracts were centrifuged at 3000-5000 rpm for 15 min and washed with double distilled water thrice and collected the pellet dried by using hot air oven and stored for further characterization.
The characterization of synthesized iron nanoparticles was carried out by Ultraviolet Visible (UV-VIS) absorption spectrophotometer and Scanning Electron Microscopy (SEM). Synthesized extracts of 0.3 ml of iron nanoparticle solution were diluted in 3 ml distilled water. UV-VIS spectroscopy analysis was carried out on a Jasco v 530, UV-VIS absorption spectrophotometer with a resolution of 2.0 nm between 200 to 800 nm possessing a scanning speeds of 300 nm per min. The process of reaction between metal ions and biosynthesis of iron nanoparticle was monitored by UV-VIS spectra of iron nanoparticles in aqueous solution. Synthesized extracts were centrifuged at 3000-5000 rpm for 15 min and washed with double distilled water thrice and collected the pellet. The pellet was dried by using hot air oven and stored for further characterization. For SEM, analysis the Iron nanoparticle synthesized by using mushroom was allowed to dry completely and ground well to a powder specimen. Since, the specimen needs to be at high vacuum, fixation was usually performed by incubation in a solution of a buffered chemical fixative, such as glutaraldehyde. The dry specimen was mounted on using an adhesive epoxy resin of electrically conductive double sided adhesive tape and sputter coated with gold palladium alloy before examination in the microscope. The powdered samples were coated with gold (20 mm) palladium in a SCD 020 sputter coating machine and observed in a Tescan Vega 3, SEM at Central Instrumentation facility, The Gandhigram Rural Institute (Deemed to be University), Gandhigram, Dindigul, Tamil Nadu.
Determination of antimicrobial activities:
The microorganisms used for the study were collected from the co-culture collections of laboratory of Department of Biology, Gandhigram Rural Institute (Deemed to be University), Gandhigram, Dindigul. Gram-positive bacteria; Bacillus cereus, Staphylococcus aureus and Micrococcus mucilaginosus, gram-negative bacteria; Klebsiella pneumoniae, K. terrigena, Pseudomonas aeruginosa, Escherichia coli and Fungi; Candida albicans, C.glabrata and Candida sp. were used for the study (Table 1). The bacterial and fungal strains were maintained in nutrient agar medium. The strains were subcultured bimonthly and the cultured strains were allowed to grow for one week and stored at 5° for further analysis.
|S.No||Name of the microbial strain||Grams nature||Strain No.|
|1||Bacillus cereus||Gram-positive bacteria||GRIBIO1|
|2||Staphylococcus aureus||Gram-positive bacteria||GRIBIO5|
|3||Micrococcus mucilaginosus||Gram-positive bacteria||GRIBIO8|
|4||Klebsiella pneumoniae||Gram-negative bacteria||GRIBIO2|
|5||Pseudomonas aeruginosa||Gram-negative bacteria||GRIBIO4|
|6||Escherichia coli||Gram-negative bacteria||GRIBIO6|
|7||Klebsiella terrigena||Gram-negative bacteria||GRIBIO9|
Table 1: Standard Microbial Strains Used for Screening of Antimicrobial Activities of Pleurotus Florida Iron Nanoparticles
The iron nanoparticles synthesized using mushroom extracts were tested for antibacterial activity by agar well-diffusion method against various pathogenic gram-positive bacteria; Bacillus cereus, Staphylococcus aureus and Micrococcus mucilaginosus, gram-negative bacteria; Klebsiella pneumoniae, K. terrigena, Pseudomonas aeruginosa, Escherichia coli and Fungi; Candida albicans, C. glabrata and Candida sp. Nutrient agar (NA) plates were spread with the help of L-rod inside the laminar air flow chamber with 8 h old broth culture of respective bacteria and fungi. The wells with 6 mm diameter and about 2 cm a part were made in a medium of petriplate each using sterile cork borer. Mushroom nanoparticle sample was prepared at a different concentration (100, 150 and 200 mg/ml and added with the help of micropipette into the wells and allowed to diffuse at room temperature for 2 h. Standard antibiotic, Ampicillin (both bacteria and fungi) was (100 mg/ml) selected to serve as positive control. Control experiments comprising inoculums without mushroom nanoparticle sample were set up and the plates were incubated at 37° for 18-24 h for bacterial pathogens and 28° for 48 h for fungal pathogens[13,14,15]. The diameter of the inhibition zone (mm) was measured by Antibiotic scale and the activity index was also calculated for triplicates of each experiment.
Results and Discussion
Different concentration of 1 M Ferric chloride solutions (10 ml -50 ml) were prepared and P. florida extract were added (50 ml). After adding the mushroom extracts with Ferric chloride solution, the colour of the solution has turned from brown to dark brown which indicated the formation of iron nanoparticles in 1:1 ratio. The synthesized iron nanoparticle extracts were centrifuged at 3000-5000 rpm for 15 min and washed with double distilled water thrice and collected the pellet. The pellet was dried by using hot air oven and stored for further characterization.
Synthesized extracts of 0.3 ml of iron nanoparticle solution was diluted into 3 ml by distilled water. UV- VIS spectral analysis was done by using UV-VIS spectrophotometer Systronics 118 at the range of 200-800 nm. The intensity of peak with respect to the height, increased gradually with increase of time. It was observed from the spectra that the iron surface plasma band occurs at 270 nm. It is well known that, the size and shape of iron nanoparticles reflect the absorption peak. The position of the peak strongly depends on the shape and size of the nanoparticles in association with other parameters which are identical to the characteristics of UV VIS spectrum of metallic iron and it was recorded (Fig. 2).
SEM technique was employed to visualize the size and morphology of nanoparticles. Most of the iron nanoparticles were spherical in shape. A few agglomerated iron nanoparticles were also observed in some places. The morphology and the particle size of iron nanoparticles were determined by using SEM micrography. Synthesized nanoparticles were spherical in size and roughly with 100 nm. Morphological studies of the synthesized iron nanoparticles were carried out by using fitted with an Energy Dispersive Spectrophotometer (EDS) (Fig. 1C-F & Fig. 3).
The sample was observed at 10,000X magnification with an accelerating voltage of 20 kV. The energy dispersive X-ray analysis (EDX) revealed strong signal in the maximum percentage (87.57 %) of iron (Fe) region was found in synthesized powder sample and it was confirmed with the formation of iron nanoparticles. The other peak for Chlorine (2.42 %), Sodium (3.20 %) and Oxygen (6.81%) in Fe is due to the precursors of iron nanoparticles (Fig. 3).
The iron nanoparticles synthesized using mushroom extracts were tested for antimicrobial activity by agar well-diffusion method against various pathogenic gram-positive bacteria; Bacillus cereus, Staphylococcus aureus and Micrococcus mucilaginosus, gram-negative bacteria; Klebsiella pneumoniae, K. terrigena, Pseudomonas aeruginosa, Escherichia coli and Fungi; Candida albicans, C. glabrata and Candida sp. and the zone of inhibition was measured in mille meter (mm). In addition, the inhibition zones formed by standard antibiotics (Amphicillin) and those of negative controls were recorded. Among these different concentrations (100 µg/ml, 150 µg/ml and 200 µg/ml), the iron nanoparticles of P. florida were exhibited antimicrobial activity against all the selected microorganisms. The clear inhibition zones were found at 24 h after incubation at 37°.
The iron nanoparticles of Pleurotus florida showed strong inhibition (200 µg/ml) against Candida glabrata (20±1.57 mm) and lower activity was found in Staphylococcus aureus (16±1.50 mm). The zone of inhibitions was ranging from 20-24 mm diameter in 200 µg/ml concentration. The highest zone of inhibitions (20±1.57 mm) was noted in Candida glabrata followed by Micrococcus mucilaginosus (20±1.13 mm), Pseudomonas aeruginosa (19±0.96 mm), Candida albicans (19±0.87 mm), Klebsiella terrigena (18±1.39 mm), Escherichia coli (17±1.32 mm), Candida sp. (17±1.23 mm), Klebsiella pneumonia (17±0.51 mm), Bacillus cereus (17±0.37 mm) and Staphylococcus aureus (16±1.50 mm) (Table 2; Fig. 4). The iron nanoparticles of P. florida showed highest zone of inhibition ranging from 14-17 mm diameter in 150 µg/ml concentration. The highest zone of inhibitions (17±1.93 mm) was noted in Candida glabrata followed by Micrococcus mucilaginosus (17 ±1.29 mm), Pseudomonas aeruginosa (17±1.15 mm), Candida albicans (15±1.81mm) Klebsiella terrigena (15±1.15 mm) Bacillus cereus (14±1.70 mm) Candida sp. (14±1.53 mm) Klebsiella pneumoniae (14±1.31 mm) Staphylococcus aureus (14±0.81 mm) and Escherichia coli (14±0.81 mm) at 150 µg/ml concentration (Table 2; Fig. 4).
|S.No||Name of the microbial strains||Zone of inhibition(mm)|
|100 µg/ml||150 µg/ml||200 µg/ml||Control (FeCl3)||Ampicillin
Table 2: Effect of Iron Nanoparticles of Pleurotus Florida on Zone of Inhibition against Selected Pathogenic Microorganism
The iron nanoparticles extract (100 µg/ml) of P. florida showed higher zone of inhibition against Pseudomonas aeruginosa (14±1.67 mm) followed by Candida glabrata (14±0.31 mm), Candida albicans (13±1.97 mm), Micrococcus mucilaginosus (13±0.76 mm), Staphylococcus aureus (12 ±1.73 mm), Klebsiella terrigena (12±1.43 mm), Candida sp. (12±1.31 mm), Bacillus cereus (12±0.96mm), Escherichia coli (12±0.57mm) and Klebsiella pneumoniae (11±0.31mm) (Table 2; Fig. 4).
Among these three concentrations (100 µg/ml, 150 µg/ml and 200 µg/ml) of iron nanoparticles, the higher concentration (200 µg/ml) showed higher inhibitory activity against all the tested microorganisms. The negative control of Ferric chloride had lower effect (5-7 mm) on microbial growth whereas the positive control (Amphicillin) has showed a strong inhibition of selected microorganisms in the ranging from 20-21 mm diameter in 100 µg/ml concentration. Interestingly, the lower concentration (100-150 µg/ml) of iron nanoparticles extract of mushroom were equally worked against the pathogenic microorganism more than that of standard antibiotic (Ampicillin) used. Generally, Ampicillin at 100 µg/ml alone inhibiting the pathogenic fungi at the range of 20-21 mm, whereas, the lower concentration of (100-150 µg/ml) iron nanoparticles extract equally responded against pathogenic microbes.
Nanoparticles have many effects on human health relative to bulk material from which they are produced. The increase in the biological activity of nanoparticles can be beneficial, detrimental or both and nanoparticles are generally small enough to have an access to skin, lungs, and brain. Chemical synthesis methods lead to presence of some toxic chemicals absorbed on the surface that may have adverse effect in the medical application. Biological synthesis provides advancement over the chemical and physical method as it is coast effective, environmental friendly, easily scaled for large scale synthesis and no need to use high pressure, energy, temperature and toxic chemicals. Moreover, the utilization of biological systems through nanoparticles synthesis is a notable alternative in advanced multifaceted approaches.
In previous research, most of the researchers have focused the synthesis of nanoparticles from mushroom extract with silver and no works have been attempted by iron particles. It is a first attempt to study the synthesis of iron nanoparticles from extract of P. florida. Pleurotus florida extract was used for synthesis of iron nanoparticles in different ratio of Ferric chloride solutions and different ratio of mushroom extracts were added into Ferric chloride solution (1:1 ratio). It has turned from brown to dark brown colour which indicated the formation of iron nanoparticles. The development of dark brown colour of the nanoparticles is due to the surface plasmon resonance (SPR) exhibited by nanoparticles. The iron nanoparticles were spherical with increased productivity as shown by sharp and intense surface Plasmon resonance bonds for the nanoparticles prepared using an excess of the extract. The synthesized iron nanoparticles were characterized by UV- VIS Spectroscopy which has proved to be very useful for the analysis of nanoparticles. The intensity of peak with respect to the height, increased gradually with increase of time. It was observed from the spectra that the iron surface plasma band occurs at 270 nm.
The morphology and the particle size of iron free were determined by using SEM. The Synthesized nanoparticles were spherical in size and roughly with 100 nm. Similar yields and shape of silver nanoparticles were reported in earlier studies of Jaidev and Narasimha with Agaricus bisporus and P. florida mushrooms. The EDX has revealed strong signal in maximum percentage (87.57 %) of iron (Fe) region which was found in synthesized powder sample and it was confirmed with the formation of iron nanoparticles. The other peak for Chlorine (2.42 %), Sodium (3.20 %) and Oxygen (6.81 %) in Fe has due to the precursors of iron nanoparticles.
In the present study, the iron nanoparticles synthesized by using mushroom extracts were tested for antimicrobial activity by agar well-diffusion method against various pathogenic Gram-positive bacteria, gram-negative bacteria and Fungi. The iron nanoparticles of P. florida showed strong inhibition against Candida glabrata and lower activity was found in Staphylococcus aureus. To determine the antibacterial activity of silver nanoparticles, against bacterial strains such as E.coli, Staphylococcus sp., Pseudomonas sp., and Bacillus sp., the cultures were grown overnight in nutrient broth on a rotary shakers (200 rpm) at 37° and inoculated into nutrients agar plates. Different concentrations (10 µl, 50 µl, 100 µl) of sample were poured to the agar wells. The plates were incubated at 37° for 24 h. After the period of incubation, zone of inhibition around the wells were measured.
Smania et al.[20,21] proved that this mushroom produces cinnabarine, an orange pigment active against Bacillus cereus, Staphylococcus aureus, Leuconostoc mesenteroides, Lactobacillus plantarum and several Streptococcus spp. Cinnabarine was more active against gram-positive than gram-negative bacteria.
Ozen et al. and Tambeker et al. reported positive report against Escherichia coli, but also against Pseudomonas aeruginosa, Enterobacter aerogenes, Klebsiella pneumoniae, Proteus vulgaris, Salmonella typhi and Salmonella typhimurium. However, these divergences may be due to different methods and concentrations used. The methanolic extracts of Agaricus bitorquis were effective against three of gram negative bacteria namely Yersinia enterocolitica, Klebsiella pneumoniae and Proteus vulgaris. A. essettei, A. silvicola, A. silvaticus and A. nigrecentulus were not showed any antibacterial activity against gram negative bacteria.
It is a first attempt on synthesize of iron nanoparticles by using mushroom extract. The production of iron nanoparticles from Pleurotus florida was initially con- firmed by color changes from brown to dark brown and were further characterized by UV-VIS spectroscopy and SEM. The biosynthesized iron nanoparticles showed higher zone of inhibition against Candida glabrata fol- lowed by Micrococcus mucilaginosus, Pseudomonas aeruginosa, Candida albicans, Klebsiella terrigena, Escherichia coli, Candida sp., Klebsiella pneumoni- ae, Bacillus cereus and Staphylococcus aureus. This extract was effectively tested against microorganisms which was well comparable with standard antibiotics. The present study has recommended iron nanoparti- cles from mushrooms have great potential to act as safe alternative to antibiotics to fight the challenges of drug discovery.
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