Comparison of Umbelliprenin and Auraptene in Cytotoxic Effects and Myeloid Cell Leukemia Type-1 (Mcl-1) Gene Expression
Cellular and Molecular Research Center, 1Department of Physiology and Pharmacology, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran
Department of Physiology and Pharmacology, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran E-mail: [email protected]
Induction of apoptosis in tumor cells is one of the mechanisms of chemotherapy. Myeloid cell leukemia type 1 is one of the Bcl-2 protein families that make an important role in chemo resistance to drugs. Thus down-regulation of myeloid cell leukemia type 1 gene is one of the aims in chemotherapy. Umbelliprenin and auraptene are naturally coumarins. Cytotoxicity and apoptosis induction is one of their effects. In this study we examined the cytotoxicity of these two coumarins against some cancerous cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay method and compare them together. In next step, we tested the effect of auraptene. On myeloid cell leukemia type 1 gene expression in Jurkat cells by real time polymerase chain reaction method. We found that umbelliprenin and auraptene. Have cytotoxic effect against cancerous cell lines. Auraptene is more cytotoxic than umbelliprenin. We also found that auraptene down-regulates myeloid cell leukemia type 1 gene expression in a pattern that different from umbelliprenin.