Evaluation of Total Phenolic Compound and Cytotoxic Activity of Clinacanthus nutans
Department of Pathobiology, Faculty of Science, Mahidol University, Ratchathewi, Bangkok 10400, Thailand
Wannee Jiraungkoorskul Department of Pathobiology, Faculty of Science, Mahidol University, Ratchathewi, Bangkok 10400, Thailand E-mail: [email protected]
Clinacanthus nutans leaves were extracted in distilled water at 0.5, 1, 3, 5 and 24 h. The plant extraction were determined the highest amount of total phenolic compound and used for evaluating the cytotoxicity test. The median (LC50) and 90% lethal concentration (LC90) against Artemia salina at varied concentrations of this plant extractions as 0, 400, 800, 1600, 3200, 6400 and 12800 µg/ml were evaluated within 24 h exposure. The result revealed that the total phenolic compound measurements in each time extraction were 34.68±3.17, 46.71±9.31, 36.13±7.06, 26.53±8.83 and 30.15±3.09 mg gallic acid equivalent per g of extract, respectively. Due to the highest amount of total phenolic compound, the 1 h aqueous extract of C. nutans leaf expressed the 24 h LC50 and LC90 values against A. salina were 7,151.1 and 12,008.7 µg/ml, respectively.