All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Isolation and catalytic competence of different animal liver microsomal fractions prepared by calcium-aggregation method

Author(s): Pradnya S Walawalkar, Pooja S Serai, KR Iyer
Department of Pharmaceutical Chemistry, Bombay College of Pharmacy, Kalina, Mumbai-400 098, India

Correspondence Address:
K R Iyer Department of Pharmaceutical Chemistry, Bombay College of Pharmacy, Kalina, Mumbai-400 098 India E-mail: [email protected]


Rat, mouse, rabbit, and guinea pig liver microsomes were prepared, using the calcium-aggregation method. The mean specific spectral cytochrome P450 content obtained for rat, mouse, rabbit, and guinea pig liver microsomes were 0.547, 0.394, 0.677, and 0.378 nmol cytochrome P450/mg protein, respectively. All the microsomal samples showed the ability to biotransform p-nitrophenol to p-nitrocatechol. The mean rate of formation of p-nitrocatechol by rat, mouse, rabbit, and guinea pig liver microsomes was 0.272, 0.247, 0.497 and 0.424 nmole/min/nmole cytochrome 450, respectively. This method circumvents the need of an ultracentrifuge for isolation of microsomes, by the conventional differential centrifugation method.



Share this