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Abstract

Antioxidant, Lipid Peroxidation and Molecular Docking Investigations of Solvent Extracts of Argyreia imbricata

Author(s): Gowri Thippeswamy Megha, H. U. Pannagashayana, C. K. Gurumurthy, C. K. Sumachirayu, R. Hemagirigowda, R. Achur and N. Shivaiah*
Department of Biochemistry, Kuvempu University, Shankaraghatta, Karnataka 577451, 1Department of Studies and Research in Botany, 2Department of Studies and Research in Biochemistry, Tumkur University, Tumkur, Karnataka 572103, 3Department of Biological Sciences, Jnana Bharathi Campus, Bangalore University, Bangalore, Karnataka 560056, India

Correspondence Address:
N. Shivaiah, Department of Studies and Research in Biochemistry, Tumkur University, Tumkur, Karnataka 572103, India, E-mail: nagarajubiochem@gmail.com


Argyreia imbricata belongs to the family Convolvulaceae and is commonly referred as imbricate wattle. The plant has been known to exhibit in vitro and in vivo anti-diabetic activities. In this study, we have prepared the leaves extract using three different solvents methanol, ethyl acetate, and chloroform separately by the Soxhlet extraction method. The extracts were analyzed for phytoconstituents by gas chromatography-mass spectrometry. The extracts were studied for total antioxidant activity, ferric reducing antioxidant power assay, 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, 2,2-azino-bis-3-ethylbenzthiazoline-6- sulphonic acid radical cation decolorization assay, hydrogen peroxide scavenging assay and lipid peroxidation activities. Molecular docking was used to further study interaction of the phytoconstituents. L-ascorbic acid is used as a standard antioxidant in all the antioxidant assays. Among the three extracts, methanol extract showed the highest antioxidant activity, as measured by total antioxidant activity of 1.231μg/ml and ferric reducing antioxidant power of 14.9 μg/ml. Furthermore, methanol extract showed significant 1,1-diphenyl-2- picrylhydrazyl radical scavenging activity with an half maximal inhibitory concentration value of 7.06 μg/ml, 2,2-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid radical cation decolorization assay with a value of 13.91 μg/ml, hydrogen peroxide scavenging assay with a value of 19.71 μg/ml and inhibited of lipid peroxidation with half maximal inhibitory concentration value of 128.4 μg/ml. The presence of different phytoconstituents was analyzed by gas chromatography-mass spectrometric analysis. The potential interactions between identified phytoconstituents and the antioxidant target protein were studied using docking study. The phytoconstituents of methanol extract scored the highest interaction with 2HE3. This study focuses on the pharmacological discovery process for preventing cell damage by oxidants.

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