Augmenting Bioactive Metabolite Synthesis from Streptomyces Species Isolated from Mangrove Sediments through Physio-Chemical Parameters
Research and Development Centre, Bharathiar University, Coimbatore 641046, 1Department of Biotechnology, K S Rangasamy College of Arts and Science (Autonomous), Tiruchengode 637215, 2Department of Botany, Arignar Anna Government Arts College, Namakkal 637002, Tamil Nadu, India
B. Veluchamy, Department of Botany, Arignar Anna Government Arts College, Namakkal 637002, Tamil Nadu, India, E-mail: email@example.com
Actinomycetes were isolated from the sediment samples collected from Pichavaram Mangrove forest in Thandavarayan Sholagan Pet, Chidambaram Taluk, Cuddalore district, Tamilnadu, South India. The actinomycetes were screened for antibacterial (bioactive metabolite) production against Gram positive and Gram negative bacterial organisms. Molecular characterization was performed through 16S rRNA gene amplification to identify the actinomycetes. Standardization of media was done by designing the media at various pH, incubation temperature and sodium chloride concentration and incubation time. It is concluded that four actinomycetes were able to synthesis bioactive metabolite and two actinomycetes (PMA2 and PMA6) were characterized at molecular level as Streptomyces species. They grow better at pH 7.0 and the optimum temperature was found to be 34-37°. PMA2 tolerates up to 5 % sodium chloride concentration, whereas PMA6 tolerates up to 7.5 %. Minimum 7 d of incubation time is necessary for the growth of actinomycetes and concurrently, the antibacterials were peaked from 8th d.