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Abstract
Development and validation of a simultaneous HPLC method for estimation of bisoprolol fumarate and amlodipine besylate from tablets
Author(s): DN Vora, AA Kadav
Chemistry Dept, Chauhan Institute of Science, Mumbai, India
Correspondence Address:
DN Vora Chemistry Dept, Chauhan Institute of Science, Mumbai India E-mail: dn.vora@rediffmail.com
Chemistry Dept, Chauhan Institute of Science, Mumbai, India
Correspondence Address:
DN Vora Chemistry Dept, Chauhan Institute of Science, Mumbai India E-mail: dn.vora@rediffmail.com
A fast, robust and stability indicating RP-HPLC method was developed for simultaneous determination of bisoprolol fumarate and amlodipine besylate in tablets. The mobile phase was mixture of 25 mM ammonium acetate adjusted to pH 5.0 and methanol (65: 35) at 0.8 ml/min. The stationary phase was Luna C18-2 column (3 μ, 50×4.6 mm ID). UV detection was performed at 230 nm. Retention time was 1.45 min and 3.91 min for bisoprolol and amlodipine, respectively. Linearity was established in the range of 8–33 μg/ml. Mean recovery was 99.1% and 98.6% for bisoprolol fumarate and amlodipine besylate, respectively.
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