Abstract
Development and validation of reverse-phase high-performance liquid chromatography method for estimation of citicoline sodium in bulk and dosage form
1Department of Pharmaceutical Quality Assurance, Veerayatan Institute of Pharmacy, Jakhania-370 460, India 2Department of Pharmaceutical Quality Assurance, N.R.Vekaria Institute of Pharmacy and Research Centre, India
Correspondence Address:
H K Maradiya Department of Pharmaceutical Quality Assurance, Veerayatan Institute of Pharmacy, Jakhania-370 460 India E-mail: hitu.pharma87@gmail.com
A simple, accurate, precise and sensitive reverse-phase high-performance liquid chromatography method for the determination of citicoline sodium has been developed and validated. Drug was resolved on a C18 column (Phenomenex Luna, 250×4.6 mm, 10μ), utilizing mobile phase of potassium dihydrogen phosphate buffer and acetonitrile in a ratio of 30:70. Mobile phase was delivered at the flow rate of 1.0 ml/min and detection was carried out at 272 nm. Separation was completed within 2.22 min. Calibration curve was linear with good correlation coefficient (R 2 =0.999) over a concentration range 10-60 μg/ml. Recovery was between 98.84 and 101.76%. Method was found to be reproducible with relative standard deviation for intra and interday precision of <2.0% over the said concentration range. The method was successfully applied to the determination of the citicoline sodium, it can be very useful and an alternate to performing the stability studies.
