Abstract

To determine how butein affects the oxidative harm caused by high glucose levels on human retinal vascular endothelial cells, as well as its method of action in doing so (human retinal vascular endothelial cells). The high glucose group, high glucose+butein-low group, high glucose+butein-middle group, high glucose+buteinhigh group, control group, high glucose+microRNA-negative control group, high glucose+microRNA- 146a group, high glucose+butein+anti-microRNA-negative control group, and high glucose+butein+antimicroRNA- 146a group were each given their own group of human retinal vascular endothelial cells. We used flow cytometry, the dichloro-dihydro-fluorescein diacetate method and colorimetric methodology to assess the apoptotic rate, reactive oxygen species level, superoxide dismutase activity, and malondialdehyde content of human retinal vascular endothelial cells. To determine the expression of microRNA-146a, real time-qualitative polymerase chain reaction was employed. Superoxide dismutase activity and microRNA- 146a expression were both substantially lower (p<0.05) in the high glucose group than in the control group, although the apoptotic rate, reactive oxygen species level, and malondialdehyde content of human retinal vascular endothelial cells in the high glucose group were all considerably greater (p<0.05) than those in the control group. In contrast to the high glucose group, the high glucose+butein-low, high glucose+buteinmiddle, and high glucose+butein-high groups significantly reduced the apoptotic rate, reactive oxygen species level, and malondialdehyde content of human retinal vascular endothelial cells while significantly increasing superoxide dismutase activity and microRNA-146a expression (p<0.05). In comparison to the high glucose+microRNA-negative control group, the apoptotic rate, reactive oxygen species level and malondialdehyde content of human retinal vascular endothelial cells in the high glucose+microRNA-146a group were all substantially lower (p<0.05), and superoxide dismutase activity was also considerably greater (p<0.05). The apoptosis rate, reactive oxygen species level, and malondialdehyde content of human retinal vascular endothelial cells in the high glucose+butein+anti-microRNA-146a group were noticeably greater (p<0.05) than in the high glucose+butein+anti-microRNA-negative control group, whereas the superoxide dismutase activity was noticeably lower (p<0.05) in comparison. Butein may be able to lessen the oxidative harm than high glucose levels due to human retinal vascular endothelial cells by increasing the expression of microRNA-146a.