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Effect of Ginsenoside Rd on the Biological Characteristics of Lung Adenocarcinoma H1299 Cell Line

Author(s): Wei Ding, Xudong Qin, Lingjie Zhou and Zhixiang Ming*
Department of Thoracic Surgery, 1Department of General Surgery, Nantong Hospital of Traditional Chinese Medicine, Nantong, Jiangsu Province 226001, China

Correspondence Address:
Zhixiang Ming, Department of General Surgery, Nantong Hospital of Traditional Chinese Medicine, Nantong, Jiangsu Province 226001, China, E-mail:

To explore the effect of ginsenoside Rd on the biological characteristics of lung adenocarcinoma H1299 cell line. Firstly, 10 μmol/l ginsenoside Rd, 20 μmol/l ginsenoside Rd, 40 μmol/l ginsenoside Rd, 80 μmol/l ginsenoside Rd, 160 μmol/l ginsenoside Rd were applied to treat H1299 cell line. 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide assay was used to measure the inhibitory rate of glutathione reductase on cell viability, and the half-maximal inhibitory concentration value was calculated as 48.17 μmol/l. After treating H1299 cell line with 48.17 μmol/l ginsenoside Rd for 3 d, effects of ginsenoside Rd on proliferation ability, migration ability, and apoptosis were respectively detected using Ki67 immunofluorescence, Transwell culture system, and terminal deoxynucleotidyl transferase dUTP nick end labeling assay kit. Ginsenoside Rd had inhibitory effects on the viability of H1299 cells, and the inhibition is concentration-dependent. The half-maximal inhibitory concentration value is 131.92 μmol/l; compared to the control group, treatment of H1299 cells with 130 μmol/l ginsenoside Rd induced a significant decrease in Ki67-positive cells number and migratory cells. However, there was hardly any observed apoptosis in both groups. Ginsenoside Rd can suppress activity, proliferation ability, and migration capability of H1299 cell line, which indicates a noticeable suppression of malignancy in H1299 cells. Ginsenoside Rd may have potential therapeutic effects in the treatment of lung adenocarcinoma.

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