Abstract
Effects and Mechanism of Periplaneta americana Extract on the Improvement of Bone Marrow Hematopoietic Function During Liver Cancer Chemotherapy
College of Pharmacy Dali University, 1Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical Research and Development, Dali University, Dali, Yunnan Province 671000, China
Correspondence Address:
Mexian Guo*, Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical Research and Development, Dali University, Dali, Yunnan Province 671000, China, E-mail: yndllyo@126.com
Periplaneta americana is an insect belonging to the Insectoptera family Blattella. It is one of the oldest and most fertile insect groups. In China, dried extract of Periplaneta americana has been used to treat blood stasis, acne and abdominal mass for a long time. In this study, we will explore the effect of Periplaneta americana extract on the improvement of hematopoiesis damage during cyclophosphamide treatment and its potential mechanism. Firstly, we established H22 tumor-bearing mice, gave different doses of CII-3 then quantified the count of bone marrow nucleated cell in femur and peripheral blood cells. Furthermore, we measured the thymus index and bone marrow cell cycle of the mice, and pathological analysis of femur was performed by hematoxylin and eosin staining. Finally, using real-time polymerase chain reaction to detect the messenger ribonucleic acid expression of intercellular cell adhesion molecule-1 and transforming growth factor-beta1. Periplaneta americana extract notably increase the number of bone marrow nucleated cell and peripheral blood cells, increase level of hemoglobin, raise the thymus index, induce bone marrow cells to reenter the proliferation cycle, increase the proliferation index and the messenger ribonucleic acid expression of intercellular cell adhesion molecule-1 and decrease the messenger ribonucleic acid expression of transforming growth factorbeta 1. This study found that Periplaneta americana extract can significantly improve the hematopoietic impairment induced by cyclophosphamide in tumor-bearing mice. These effects may be mediated by the increase of intercellular cell adhesion molecule-1 and decrease of transforming growth factor-beta 1.
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