Abstract

To explore the influence and possible mechanism of extracts from Phellinus igniarius (expanded program on immunization) on oral squamous cell carcinoma cell proliferation, migration and invasion. CAL27 cells were treated with expanded program on immunization or transfected with microRNA- 655-3p. Furthermore, transfected CAL27 cells were exposed to 600 μg/ml expanded program on immunization. Proliferation, migration and invasion were assessed using 3-(4,5-dimethylthiazole-2-yl)- 2,5-diphenyltetrazolium bromide and Transwell. Matrix metallopeptidase 2, matrix metallopeptidase 9 and proliferating cell nuclear antigen protein levels were monitored using Western blot. After various doses of expanded program on immunization, cell viability, migration, invasion, proliferating cell nuclear antigen, matrix metallopeptidase 2 and matrix metallopeptidase 9 protein levels were reduced, but microRNA-655-3p content was enhanced in a dose-dependent way. Meanwhile, increased microRNA-655- 3p might diminish cell proliferation, migration and invasion. Beyond that, microRNA-655-3p knockdown overturned high-dose expanded program on immunization-mediated tumor development repression. Expanded program on immunization could hinder oral squamous cell carcinoma cell development via modulating microRNA-655-3p.