Abstract
Evaluation of the Effect of Quercetin on Microcirculation Perfusion in Renal Injury of Rats with Ischemia Reperfusion Injury by Contrast-Enhanced Ultrasound
North Sichuan Medical College, Nanchong 637000, 1Department of Ultrasound, The First Medical Center, Chinese PLA General Hospital, Beijing 100853, China
Correspondence Address:
Yukun Luo, Department of Ultrasound, The First Medical Center, Chinese PLA General Hospital, Beijing 100853, China, E-mail: lykun1123@163.com
To explore the application value of contrast-enhanced ultrasound to evaluate the changes in blood microcirculation in the renal cortex during renal ischemia reperfusion injury in rats. 18 Sprague-Dawley rats were randomly divided into control group (sham group), renal ischemia reperfusion group (ischemia-reperfusion injury group) and quercetin intervention group (ischemia-reperfusion injury+quercetin group), with 6 rats in each group. Ischemia-reperfusion injury+quercetin group was given 100 mg/kg quercetin by intragastric administration 7 d before surgery, and all three groups underwent contra-enhanced ultrasound examination 24 h after surgery. Blood was collected from the inferior vena cava, and creatinine and urea nitrogen levels were detected by automatic biochemical analyzer. The levels of interleukin-6, tumor necrosis factor-alpha and interleukin-10 were detected by enzyme-linked immunosorbent assay kit. The left kidney tissue of rats was collected and periodic acid-Schiff staining was performed for pathological detection. In terms of inflammatory factors, compared with sham group, interleukin-6, tumor necrosis factor-alpha and interleukin-10 in ischemia-reperfusion injury group were significantly increased (p<0.05), while interleukin-6 and tumor necrosis factor-alpha were significantly decreased and IL-10 was significantly increased in ischemia-reperfusion injury+quercetin group compared with ischemia-reperfusion injury group (p<0.05). Pathologically, a large number of tubular shapes were observed in the renal interstitium of rats in the ischemia-reperfusion injury group, during which inflammatory cell infiltration was observed. The injury degree of ischemia-reperfusion injury+quercetin group was lower than that of the ischemia-reperfusion injury group and the tubular shapes were not obvious. Contrast-enhanced ultrasound can evaluate the changes of blood flow microcirculation in the renal cortex during the intervention of quercetin in renal ischemia-reperfusion injury in rats. Quercetin can improve the renal cortical microcirculation and reduce the expression of inflammatory factors during renal ischemia reperfusion injury in rats.
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