Fractional Separation Assisted Ultra Violet Spectrophotometric Determination of Oleanolic Acid in Eugenia caryophyllus (Spreng.)
Department of Pharmaceutical Sciences, Shri Rawatpura Sarkar Institute of Pharmacy, Kumhari, Chhattisgarh 490042, 1Department of Pharmacognosy and Phytochemistry, Apollo College of Pharmacy, Anjora, Chhattisgarh 491001, India
Nilesh Banarase, 1Department of Pharmacognosy and Phytochemistry, Apollo College of Pharmacy, Anjora, Chhattisgarh 491001, India, E-mail: email@example.com
Ultraviolet spectrophotometry has been successfully used for the qualitative as well as quantitative determination of various phytoconstituents. For the quantitative determination of phytoconstituents in the extract by ultraviolet spectrophotometry, the absorbance at specific wavelength is measured. As extract is a mixture of number of constituents, the absorbance of different constituents at the same wavelength along with the compound of interest can occur giving wrong interpretation of observed data and subsequently the results. So, in order to overcome said problem, the technique of fractional separation was used to remove the interfering chemical constituents at the wavelength of compound of interest. In this study, we quantitatively determined the oleanolic acid as a compound of interest in Eugenia caryophyllus flower buds using ultraviolet spectrophotometry.