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Houttuyn Berberine Promotes Apoptosis of Nasopharyngeal Carcinoma Cells and Inhibits Proliferation, Migration and Invasion by Regulating the circ-SMARCA5/microRNA-346 pathway

Author(s): Dongbao Yang, Xiaodong Wen, Jing Xiao, Hong Qin, Jie Hou, Yujie Li, Xiaojun Liang1 and Fuyu Qiao*
Department of Otolaryngology-Head and Neck surgery, Fengdu People’s Hospital of Chongqing, Dadukou, 1Department of Otolaryngology-Head and Neck surgery, The Second Affiliated Hospital of The Army Medical University, Fengdu, Chongqing 400084, China

Correspondence Address:
Fuyu Qiao, Department of Otolaryngology-Head and Neck surgery, Fengdu People’s Hospital of Chongqing, Dadukou, Chongqing 400084, China, E-mail:

To explore the effect of houttuyn berberine on the biological behavior of nasopharyngeal carcinoma cells by regulating circ-SMARCA5/microRNA-346 pathway. Nasopharyngeal carcinoma cells C666-1 were divided into control group, houttuyn berberine (1, 2, 4 μg/ml) group, plasmid cloning deoxyribonucleic acid group-circSMARCA5 group, houttuyn berberine+si-circ-SMARCA5 group, and houttuyn berberine+microRNA-346 group. Real-time quantitative polymerase chain reaction detected the expression levels of circ-SMARCA5 and microRNA-346. Cell counting kit, plate cloning experiment, flow cytometry, wound healing assays and Transwell assays were performed to assess the cell viability, clone formation, apoptosis, migration and invasion ability of C666-1. The targeting relationship between circ-SMARCA5 and microRNA-346 was verified with the aid of dual luciferase experiment. In comparison with the control group, the cell viability of C666-1 cells, microRNA-346 expression, wound healing rate, clone formation numbers, invasive numbers in the houttuyn berberine (1, 2, 4 μg/ml) group were increased, and the expression of circ-SMARCA5 and apoptosis rate were increased (p<0.05). Compared with plasmid cloning deoxyribonucleic acid group, the cell viability of C666-1 cells, wound healing rate, microRNA-346 expression in the plasmid cloning deoxyribonucleic acidcirc-SMARCA5 group were reduced, the apoptosis rate was increased, invasive numbers and clone formation numbers were reduced (p<0.05). Circ-SMARCA5 directly bound to microRNA-346. Compared with the houttuyn berberine group, the cell viability of C666-1 cells, clone formation numbers and invasive numbers were increased in the houttuyn berberine+si-circ-SMARCA5 group and houttuyn berberine+microRNA-346 group, the apoptotic rate was decreased, and the wound healing rate was increased (p<0.05). Houttuyn berberine could inhibit nasopharyngeal carcinoma cells’ malignant proliferation, migration and invasion, and induce cell apoptosis. The mechanism is associated with the up-regulation of circ-SMARCA5/microRNA-346 pathway.

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