Interference with Tripartite Motif-Containing Protein 26 Expression on Proliferation of Glioma Cells
Department of Neurosurgery, Affiliated 3201 Hospital of Xi’an Jiaotong University, Hanzhong, Shaanxi 723000, 1School of Foreign Studies, Shaanxi University of Technology, Hanzhong, Shaanxi 723000, 2Department of Neurosurgery, First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710061, China
Shiwen Guo, Department of Neurosurgery, First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710061, China, E-mail: email@example.com
This research aims to study the effect of interference with tripartite motif-containing protein 26 expression on the proliferation of intracranial glioma cells. This research randomly selected 60 cases of intracranial glioma specimens and the brain tissue of 10 cases of brain trauma patients as a normal control group. First, cell culture is performed to observe the growth status of the cells. Subsequently, the small interfering ribonucleic acid interference technique was used to interfere with the expression of tripartite motif-containing protein 26 on the glioma cell lines A172 and U251 cells. The cells were collected for tripartite motif-containing protein 26, Western blot and fluorescence quantitative polymerase chain reaction was used to verify the knockout effect, and the cell proliferation, clone formation, transfer, and invasion experiments were performed. Experimental data showed that among 60 glioma patients, tripartite motif-containing protein 26 samples were positive in 46 cases and 10 normal brain tissue samples were all negative. The performance of tripartite motif-containing protein 26 in glioma was compared with the performance in normal brain tissue. The difference was statistically significant (p<0.01). The results show that tripartite motif-containing protein 26 can reduce the protein level of pre-B-cell leukemia homeobox through the correlation of time gradient and concentration gradient. Tripartite motif-containing protein 26 can reduce the messenger ribonucleic acid level of ring finger protein 6 and the downstream target gene of pre-B-cell leukemia homeobox without affecting the messenger ribonucleic acid level of pre-B-cell leukemia homeobox.