Abstract

Alpha humulene, the major active ingredient of the hops plant (Humulus lupulus L.), is a monocyclic sesquiterpene composed of three isoprene units containing three unpaired C=C double bonds. Beside hops plant, it is also commonly found in herbs such as cannabis, sage, basil, and ginseng. Alpha humulene is also known as alpha Caryophyllene and it has many effects such as anti-inflammatory, antimicrobial, antioxidant, anticancer and local anaesthetic. In this study, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test was used to determine the concentration range of the cytotoxic effects of Alpha humulene in the SH-SY5Y cell line, a bone marrow derived human neuroblastoma cell line. In addition, the neuroprotective effects of Alpha humulene in the model of neurotoxicity induced by hydrogen peroxide, the half-maximal inhibitory concentration value of Alpha humulene on undifferentiated and retinoic acid induced differentiated SH-SH5Y was determined 221 μg/ml and >400 μg/ml cells using the Real-time cell analysis system. Apoptotic effect levels were determined by Acridine orange/Parkinson's disease, staining method. The SH-SY5Y cell line and the lipopolysaccharide stimulated Tohoku hospital pediatrics-1 cell line were incubated together to establish an in vitro co-culture model. In the in vitro co-culture model established, fluorescence measurements were taken in Cytation 3 multi-mode reader to determine the immunological effects of alpha humulene using lipopolysaccharide stimulation and multiple independent images were taken. The apoptotic effect caused by cellular damage caused by hydrogen peroxide alpha humulene was reduced.