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Abstract

Long Non-Coding Ribonucleic Acid Small Nucleolar Host Gene 1 Regulates the Ischemic Stroke by Targeting Micro Ribonucleic Acid-376a-Mediated Cystathionine ? Synthase/Hydrogen Sulfide Axis Pathway

Author(s): YAN HUA. WU, HUA PING. DU, YUAN. XU AND ZHI HONG. XU*
Department of Neurology, Suzhou Ninth People’s Hospital, No. 2666 Ludang Road, Suzhou, Jiangsu 215200, China

Correspondence Address:
Department of Neurology, Suzhou Ninth People’s Hospital, No. 2666 Ludang Road, Suzhou, Jiangsu 215200, China, E-mail: [email protected]


Ischemic stroke was a brain disease with high mortality and disability rate. Previous studies have shown that the expression of Micro Ribonucleic acid-376a in the serum of stroke patients was increased, suggesting that Micro Ribonucleic acid-376a was closely related to the occurrence and development of stroke. However, its mechanism has not been reported. The purpose of this study was to explore the regulatory mechanism of Long non-coding ribonucleic acid small nucleolar host gene 1 on Ischemic stroke through targeting the Micro Ribonucleic acid-376a-mediated cystathionine β synthase hydrogen sulfide axis pathway. The Ischemic stroke model of Human Cerebral Microvascular Endothelial Cell Line/D3 cells was established by the induce of Oxygen-glucose deprivation. The Micro Ribonucleic acid concentrations of small nucleolar host gene 1, Micro Ribonucleic acid-376a, and inflammatory cytokines were detected by Reverse transcription- polymerase chain reaction. Western blotting was used to detect the expression of cystathionine β synthase and apoptosis-related proteins. The apoptosis was analyzed by flow cytometry and the concentration of hydrogen sulfide was detected by the kit. The interaction between long non-coding ribonucleic acid, micro ribonucleic acid, and target genes was confirmed by the luciferase test. Our study showed that under the condition of Oxygen-glucose deprivation induction, when Micro Ribonucleic acid-376a was up-regulated, the Micro Ribonucleic acid concentration of small nucleolar host gene 1 and cystathionine β synthase in Human Cerebral Microvascular Endothelial Cell Line/D3 cells was down-regulated. Further results showed that the overexpression of Micro Ribonucleic acid-376a promoted apoptosis and inflammation, while overexpression of small nucleolar host gene 1 alleviated these processes. In terms of mechanism, Micro Ribonucleic acid-376a was the target of small nucleolar host gene 1, and cystathionine β synthase was the target of Micro Ribonucleic acid-376a. In addition, small nucleolar host gene 1 regulates the expression of cystathionine β synthase by inhibiting Micro Ribonucleic acid-376a, thus exerting its function. Long non-coding ribonucleic acid small nucleolar host gene 1 inhibits the apoptosis and inflammation of Ischemic stroke cells by inhibiting Micro Ribonucleic acid-376a and up-regulating cystathionine β synthase/hydrogen sulfide signal. These results reveal the potential mechanism of Ischemic stroke and provide a potential target for the treatment of Ischemic stroke.

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