All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Long Non-coding RNA-H19 Regulates Osteogenic Differentiation via microRNA-140-5p/Wnt1 Signaling in Bone Marrow Mesenchymal Stem Cells

Author(s): Zhengbiao Liu*, Renwei Wang, Hong Zhang, Zaiqing Zhang, Wei Liu, Reyuan Zang and Chenyu Wang
Department of Orthopedics, Suzhou Industrial Park Xinghu Hospital, Suzhou, Jiangsu Province 215000, China

Correspondence Address:
Zhengbiao Liu, Department of Orthopedics, Suzhou Industrial Park Xinghu Hospital, Suzhou, Jiangsu Province 215000, China, E-mail: xhyylzb2020@163.com


To explore the influence of the long-non coding RNA-H19/microRNA-140-5p/Wnt1 axis on the osteogenic differentiation in bone marrow mesenchymal stem cells. First, we used dual-luciferase reporter gene detection to verify long-non coding-H19/miRNA-140-5p/Wnt1 regulatory axis targeting relationship. Then long-non coding-H19 was overexpressed or silenced in bone marrow mesenchymal stem cells and then long-non coding-H19, microRNA-140-5p and Wnt1 expression were assessed using quantitative real-time polymerase chain reaction and cell activity was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide. Then the osteogenic differentiation culture was performed, formation of calcium nodules were assessed via Alizarin red staining, while the expression of osteocalcin and runt-related transcription factor 2 detected via Western blot. Wild type long-non coding RNA-H19 group and wild type-Wnt1 group showed significantly decreased luciferase activity in dual-luciferase reporter assays (p<0.05). After overexpressed long-non coding RNA-H19, the microRNA-140-5p expression decreased and Wnt1 expression increased (p<0.05), and while silenced the long-non coding RNA-H19 expression, the opposite result was found (p<0.05). In terms of cell viability, there were no significant differences between groups after long-non coding RNA-H19 was overexpressed or silenced (p>0.05). After overexpressed long-non coding RNA-H19, calcium nodules formation, mineralized bone matrix level, the expression of osteocalcin and runt-related transcription factor 2 protein significantly increased (p<0.05), and while silenced the long-non coding RNA-H19 expression, the opposite result was found (p<0.05). Long-non coding RNA-H19 overexpression promotes the bone marrow mesenchymal stem cells osteogenic differentiation via targeting microRNA-140-5p/Wnt1 signaling pathway.

Full-Text | PDF

 
 
Google scholar citation report
Citations : 69022

Indian Journal of Pharmaceutical Sciences received 69022 citations as per google scholar report