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Abstract

Luteolin Promotes Osteogenic Differentiation and Proliferation in Bone Marrow Mesenchymal Stem Cells by Up-Regulating microRNA-335-3p

Author(s): Yuanbin Zhang, Yuliang Zhang*, Xingzhong Hu, Yong Lang and Qi Sun
Department of Orthopedics, Fuyang Orthopedics and Traumatology Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang 311400, China

Correspondence Address:
Yuliang Zhang, Department of Orthopedics, Fuyang Orthopedics and Traumatology Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang 311400, China, E-mail: zyl63313718@163.com


This study aimed to investigate the impact of the luteolin-microRNA-335-3p pathway on the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells. Cell proliferation was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay and osteogenic markers were assayed by Western blotting. Luteolin treatment demonstrated a dose-dependent promotion of bone marrow mesenchymal stem cells proliferation, accompanied by an increase in osteogenic marker content within the cells. Additionally, luteolin treatment dose-dependently up-regulated microRNA-335-3p levels. The restoration of microRNA-335-3p further augmented bone marrow mesenchymal stem cells proliferation and up-regulated osteogenic differentiation markers. Conversely, knockdown of microRNA-335-3p negated the promoting effects of luteolin on bone marrow mesenchymal stem cells. Luteolin facilitates osteogenic differentiation and proliferation in bone marrow mesenchymal stem cells by elevating microRNA-335-3p. This molecular pathway holds promise as a potential therapeutic target for enhancing bone regeneration and combating bone-related disorders.

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