Mechanism of Long Non-Coding RNA MAGI2-Antisense RNA 3 targeting MicroRNA-31-5p to Regulate Proliferation and Metastasis of SiHa Cells in Cervical Cancer
Health Management Center, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, 1Department of Nursing, Huaxi Hospital of Sichuan University, Chengdu, Sichuan 610093, 2Laboratory Department, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, 3Department of Breast Surgery, Huaxi Hospital of Sichuan University, Chengdu, Sichuan 610093, China
Zhangxia Ren, Department of Breast Surgery, Huaxi Hospital of Sichuan University, Chengdu, Sichuan 610093, China, E-mail: firstname.lastname@example.org
To investigate the effect of long non coding RNA MAGI2-antisense RNA 3 on the proliferation, invasion and migration of cervical cancer cells and the underlying mechanism. SiHa cells were divided into plasmid cloning DNA group, plasmid cloning DNA-MAGI2-antisense RNA 3 group, anti-microRNA-negative control group, anti-microRNA-31-5p group, plasmid cloning DNA-MAGI2-antisense RNA 3+microRNAnegative control group and plasmid cloning DNA-MAGI2-antisense RNA 3+microRNA-31-5p group; quantitative reverse transcription polymerase chain reaction was used to determine the messenger RNA expression levels of microRNA-31-5p and MAGI2-antisense RNA 3; cell viability was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay; the number of cell migration and invasion in each group was detected by Transwell assay; dual luciferase reporter assay was performed to detect the fluorescence activity. The expression levels of microRNA-31-5p were significantly increased and those of MAGI2-antisense RNA 3 messenger RNA were decreased in cervical cancer cell lines (p<0.05); overexpression of MAGI2-antisense RNA 3 or inhibition expression of microRNA-31-5p could suppress SiHa cell proliferation, migration and invasion (p<0.05). MAGI2-antisense RNA 3 targeted regulation of microRNA-31-5p; overexpression of microRNA-31-5p partially reversed the inhibitory effects of overexpressing MAGI2-antisense RNA 3 on the proliferation, migration, invasion of SiHa cells. Long non coding RNA MAGI2-antisense RNA 3 can inhibit cervical cancer cell proliferation, migration and invasion by regulating microRNA-31-5p.