Mechanism of Regulating the Proliferation, Migration and Invasion of Cervical Cancer Hela Cells by Circular RNA Circ_0025033 Targeting microRNA 1256
Department of Pathology, School of Basic Medicine, North Sichuan Medical College, Nanchong, 637000, China
Yifan Huang, Department of Pathology, School of Basic Medicine, North Sichuan Medical College, Nanchong, 637000, China, E-mail: email@example.com
To explore the effect of circular RNA_0025033 on the proliferation, migration and invasion of cervical cancer HeLa cells and its molecular mechanism. The cancer tissues and adjacent tissues of 39 patients with cervical cancer in our hospital from January 2017 to December 2020 were selected. The expression levels of circular RNA_0025033 and microRNA-1256 were detected by Quantitative reverse transcription polymerase chain reaction. Cell counting kit 8 was used to detect cell viability. Plate cloning test was conducted to detect the number of cell clones. Scratch test was carried out to detect cell scratch healing rate. Transwell chamber was used to detect cell invasion. Western blot was adopted to detect protein expression. Dual luciferase reporter assay was carried out to detect the targeting relationship between circular RNA_0025033 and microRNA-1256. The expression level of circular RNA_0025033 in cervical cancer tissues was increased and the expression level of microRNA-1256 was decreased (p<0.05). After inhibiting the expression of circular RNA_0025033 or overexpression of microRNA-1256, cell viability was decreased and the number of cell clone formation was decreased. The healing rate of cell scratches was decreased and the number of invasive cells was decrease. E-cadherin expression was increased and N-cadherin expression was decreased (p<0.05). Circular RNA_0025033 targeted regulation of the expression of microRNA-1256 was carried out; interference with microRNA-1256 expression reversed the inhibition of circular RNA_0025033 on the proliferation, migration and invasion of cervical cancer HeLa cells. Inhibition of circular RNA_0025033 expression may inhibit the proliferation, migration and invasion of cervical cancer HeLa cells through targeted regulation of microRNA-1256.