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Abstract

Metastasis Associated Lung Adenocarcinoma Transcript 1 Inhibits Apoptosis of 661W Cells by Targeting MicroRNA-200a-3p

Author(s): Guoqiang Wu, Weibo Chen, Tong Chen, Kan Yang, Chen Li, Yanjun Yong and Jun Li*
Department of Ophthalmology, Lanzhou first people's Hospital, Lanzhou 730050, 1Department of Youth League Committee, Lanzhou Pulmonary Hospital No. 968, Lanzhou 730046, China

Correspondence Address:
Jun Li, Department of Ophthalmology, Lanzhou first people's Hospital, Lanzhou 730050, 1Department of Youth League Committee, Lanzhou Pulmonary Hospital No. 968, Lanzhou 730046, China, E-mail: [email protected]


The present study was designed to the effect of metastasis associated lung adenocarcinoma transcript 1 on apoptosis of 661W cells and to explore its role in glaucoma. A stable and metastasis associated lung adenocarcinoma transcript 1-inhibition vector and microRNA-200a-3p overexpression vector were established and transfected into 661W cells. Cell counting kit 8 and flow cytometry were employed to evaluate the proliferation and apoptosis of transfected cells and western blot was applied to measure the changes of apoptosis markers represented by B-cell lymphoma 2 associated X-protein, caspase 3 and B-cell lymphoma 2. The metastasis associated lung adenocarcinoma transcript 1 target gene was predicted and its relationship with the target gene was determined using dual luciferase reporter assay. Inhibited metastasis associated lung adenocarcinoma transcript 1 or overexpressed microRNA-200a-3p brought inhibited proliferation, increased apoptosis rate, enhanced B-cell lymphoma 2 associated X-protein and caspase-3 protein and declined B-cell lymphoma 2 protein of 661W cells. Dual luciferase reporter assay revealed the targeted binding of metastasis associated lung adenocarcinoma transcript 1 and microRNA-205. The rescue experiment found that inhibited microRNA-200a-3p expression prevented cell proliferation, apoptosis and changes in apoptosis markers caused by the inhibition of metastasis associated lung adenocarcinoma transcript 1. Metastasis associated lung adenocarcinoma transcript 1 inhibits the apoptosis of 661W cells by targeting microRNA-200a-3p, thereby preventing the progression of glaucoma. Metastasis associated lung adenocarcinoma transcript 1 is expected to be the treatment direction of this disease.

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