Abstract

Author(s): SHUANGFEI LI, YUFEI CHEN, YUE WANG, XUEWEI YANG, JING WANG, HUIRONG CHEN*
Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518071, PR China

Correspondence Address:
Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518071, PR China, E-mail: chenhr@szu.edu.cn

To provide a potential method for improving microalgal lipid production, an over expression vector that expresses lysophosphatidic acid acyltransferase gene in Phaeodactylum tricornutum was constructed. Total fatty acid production incresed significantly in over-expression transformants and the length of fatty acids ranged from C14 to C22. The response to both lysophosphatidic acid acyltransferase over- and under-expression over the growth phase was also characterized. This study indicated a potential method to increase fatty acids accumulation in microalgae by employing a metabolic engineering approach.

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