All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Over-expression of LPAAT gene in Phaeodactylum tricornutum enhances fatty acid accumulation and increases fatty acid chain length

Author(s): SHUANGFEI LI, YUFEI CHEN, YUE WANG, XUEWEI YANG, JING WANG, HUIRONG CHEN*
Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518071, PR China

Correspondence Address:
Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518071, PR China, E-mail: [email protected]

To provide a potential method for improving microalgal lipid production, an over expression vector that expresses lysophosphatidic acid acyltransferase gene in Phaeodactylum tricornutum was constructed. Total fatty acid production incresed significantly in over-expression transformants and the length of fatty acids ranged from C14 to C22. The response to both lysophosphatidic acid acyltransferase over- and under-expression over the growth phase was also characterized. This study indicated a potential method to increase fatty acids accumulation in microalgae by employing a metabolic engineering approach.

Full-Text | PDF