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Abstract

Potential Regulatory Effect of mir-182-3p on Osteosarcoma by Targeting Early B-Cell Factor 2

Author(s): H. JIANG, F. LOU, WEN GUO AND Y. LI*
Departments of orthopedics, Departments of Oncology, Taizhou People’s Hospital Affiliated to Medical College of The Nantong University, Taizhou, Jiangsu 225300, P. R. China

Correspondence Address:
Departments of orthopedics, Departments of Oncology, Taizhou People’s Hospital Affiliated to Medical College of The Nantong University, Taizhou, Jiangsu 225300, P. R. China, E-mail: [email protected]


This study investigated the role of miR-182-3p in osteosarcoma. The effect of the miR-182-3p expression on cell function and the regulatory signal changes of Early B-cell Factor 2 targeted by miR-182-3p were studied in the osteosarcoma cell line in vitro. miR-182-3p was downregulated in both osteosarcoma patients and osteosarcoma cell line. The overexpression of miR-182-3p resulted in increased apoptosis of osteosarcoma cells and decreased migration and invasion of osteosarcoma cells. In addition, miR-182-3p regulated the messenger RNA stability of early B-cell factor 2 y directly binding to the 3 prime untranslated region of Early B-Cell Factor 2, which was a key regulator of osteosarcoma cell apoptosis. Overexpression of Early B-Cell Factor 2 could inhibit the apoptosis of osteosarcoma cells and promote the migration and invasion of osteosarcoma cells effectively. At the same time, overexpression of Early B-Cell Factor 2 could alleviate the phenotype induced by miR-182-3p. miR-182-3p was a tumor suppressing microRNA in osteosarcoma. Its function was realized by inhibiting Early B-Cell Factor 2. These results provided a new therapeutic target for metastatic osteosarcoma and a better understanding of the molecular regulation of Early B-Cell Factor 2.

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