Abstract
Preparation of PEGylated Liposomal Ginsenoside;Formulation Design and in vitro Evaluation
Department of pharmaceutics, College of pharmacy, Yanbian University, 1Antu County Health Supervision Institute, 2Department of pharmacy, Yanbian University Hospital, Jilin, 133000, 3Beijing Fogangren Bio-Pharma Limited, Beijing, 102628, 4Department of Radiology, Yanbian University Hospital, Jilin, 133000, China
Correspondence Address:
Department of pharmaceutics, College of pharmacy, Yanbian University, China, E-mail: kimguangyu@163.com
In this study, ginsenoside Rg3-loaded PEGylated liposomes were prepared and optimized using the Box- Behnken design. These liposomes were characterized, the cumulative release profiles were investigated and compared with ginsenoside Rg3-loaded liposomes in vitro. To improve the stability ginsenoside Rg3-loaded PEGylated liposomes were freeze-dried and the lyoprotectants to be added were screened. The results showed that the liposomes have a small particle size (152.58±0.74 nm) and spherical shape. The encapsulation efficiency and drug-loading rate were approximately 85.24±1.02 and 7.44±0.08 %, respectively. For lyoprotectants, 2 % lactose was chosen as the lyophilized protectant according to the appearance, re-dispersity, particle size, and entrapment efficiency of lyophilization of ginsenoside Rg3- loaded PEGylated liposomes. In vitro release showed that ginsenoside Rg3-loaded PEGylated liposomes showed a more obvious sustained release effect, which suggests that ginsenoside Rg3-loaded PEGylated liposomes might enhance the therapeutic effect of ginsenoside Rg3.
