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Abstract

Reverse-phase high performance liquid chromatographic determination of Tizanidine and Valdecoxib in tablets

Author(s): CS Ramaa, DK Deshpande, AR Shirode, VV Wamorkar, AB Kakad, VJ Kadam
Department of Quality Assurance, Bharati Vidyapeeth's College of Pharmacy, Navi Mumbai-400 614, India

Correspondence Address:
C S Ramaa Department of Quality Assurance, Bharati Vidyapeeth's College of Pharmacy, Navi Mumbai-400 614 India E-mail: [email protected]


A simple, reproducible and efficient reverse-phase high performance liquid chromatographic (HPLC) method was developed for simultaneous determination of tizanidine and valdecoxib from their pharmaceutical dosage forms. Separation was done by using mobile phase of composition ammonium acetate buffer (0.1 M): methanol: acetonitrile in the ratio of 50:30:20 v/v. Quantitation was achieved with UV detection at 232 nm based on peak area, with linear calibration curves at concentration ranges from 1-100 µg/ml for tizanidine and valdecoxib respectively. The method has successfully been applied to pharmaceutical dosage forms. No chromatographic interference from the tablet excipients was found. The method was validated using ICH guidelines and was found to be highly precise, accurate and robust.

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